Radamel Falcao won't feature for Chelsea again in Europe this season after the injury-hit forward was left out of their Champions league squad.
Clubs need to provide an updated list of 22 players to be registered for the knockout stages.
New signing Alexandre Pato is included as Chelsea play French side Paris Saint-Germain in the last 16.
But being left out of Chelsea's Champions League squad is a further blow to the out-of-form forward.
The 29-year-old has just a single goal in just 11 appearances for Chelsea this season after an underwhelming spell at Manchester United last season.
Falcao had been expected to move to Atletico Madrid on Transfer Deadline Day but no deal ultimately materialised, leaving the Colombian forward stranded at Stamford Bridge where he is on loan from AS Monaco.
At his peak, he was a goal machine for Atletico Madrid and FC Porto, but a serious knee injury in early 2014 which cost him a place at the 2014 World Cup has derailed what had been a fantastic career.